( D) Schematic diagram of the domain structure of a double-tagged VHH heterodimer protein. The eight amino acids shown at the carboxyl end are encoded by either the short hinge or long hinge PCR primer that were used to generate the library. VHHs in A and B also contain Q(L/V)QLVE at the amino end that is encoded by the PCR primer used to generate the VHH-display library. ( C) Protein sequences of three VHHs recognizing the same BoNT/A epitope as ciA-H7 are shown aligned for homology. ( B) Protein sequences of VHHs recognizing unique epitopes on BoNT/B (ciB) are shown aligned for homology. ( A) Protein sequences of VHHs recognizing unique epitopes on BoNT/A (ciA) are shown aligned for homology. Figure S1: Protein sequences of anti-BoNT/A and anti-BoNT/B VHH monomers and heterodimers.
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